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Image Search Results
Journal: bioRxiv
Article Title: A workcell 1.0 for programmable and controlled operation of multiple fluidic chips in parallel
doi: 10.1101/2023.04.16.536594
Figure Lengend Snippet: Figure 2: (A-D) Schematic depicting the Load, Advance, Introduce, and Reset stages of the flow protocol that is used to move reagents from their tubes to an array of chips an arbitrary distance away, with low dead-volume. (E) Layout showing the different layers of the cooling block, which the tubes sit in while they are docked to the pressure-head. (F) Characterization of the cooling system. Temperature readings are generated by a high-precision resistance temperature detector (RTD, Pt-100) which sits in water inside of a dummy laboratory tube. (G) Chips that were used on the workcell in this study. Chip from , C, E (top), chip from (middle), chip from (bottom). (H) Demo protocol for controlling the workcell to operate chips (functional syntax is similar in simplicity). Hierarchical function definitions are shown below for “flow”, which calls “Load”, “Advance”, “Introduce”, and “Reset” functions. Functions are imported at the beginning of a protocol file. (I) Schematic overview of software architecture. The automancer software communicates by OPCUA to software and hardware running on the PLC. (J-K) Depictions of programs running on the PLC. (J) Depiction of inlet-valve pulse-width modulation (PWM) used to automate reagent mixing and on-chip dilutions. Automancer communicates to turn on PWM-mode for a specific channel, and supplies a period, and duty-cycle to the PLC, to select a specific dilution level or mixing ratio. Schematic waveforms are shown for a 40% duty cycle used to dilute orange reagent (top). An example pulse from the PLC is shown as characterized using a logic analyzer (top right). More extensive PWM characterization is shown in Supplementary Figure 3. (K) Characterization of the flowrate controller. Automancer directs the PLC to operate in flowrate-control mode, and supplies a flowrate setpoint. A characterization across a wide range of flowrates is shown in Supplementary Figure 2, and stability across resistance perturbations in .
Article Snippet: In our flow-controller we use
Techniques: Introduce, Blocking Assay, Generated, Functional Assay, Software